Paramjeet Singh Bagga,  Ph.D.

Cell and Molecular Biology (CMB): Laboratory
BIOL 406

Click on the topic-links below to open the documents containing the handouts for the listed topic. You may need a password to open some of the documents. Contact me if you need to know the password. The links will become ALIVE a few days before the scheduled lab.

Note: The links below might not be in the same order as the lab experiments are scheduled. Please look at the syllabus for correct sequence of experiments.

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Topic
1. Monday Laboratory Syllabus and Schedule of Experiments
2.

Introduction to Cell & Molecular Biology Laboratory Experiments,
Lab Safety Rules

3. How to make Molecular Biology Reagents
4. Reagent and Solutions Recipes / Culturing Recombinant E. coli clones
5. Isolation and Study of Mitochondria
6. Extraction and Analysis of Membrane Proteins
7. Extraction and Analysis of Membrane Proteins - Continued
8. Restriction Endonuclease Digestion and DNA Gel Electrophoresis

Molecular Weight Marker

Sample Results : Gel Picture
9.
10. Probe generation for Microarrays from total Yeast RNA (Will not be covered)
11.

Hybridization of cDNA probes to Yeast Microarrays

12. Analysis of Gene Expression in Yeast using Microarray Data
13. Introduction to Nucleic Acid, Protein, OMIM, PubMed and other Databases on the Internet
14. Database Search, Gene identification, Protein Structure & function. Web Design
15. Purification and Identification of Recombinant Green Flourescent Protein (GFP) Expressed in E. coli
The Final Project

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