Microcell Mediated Chromosome Transfer (MMCT) is used to transfer each human chromosome separately into the XP or CS DNA repair deficient cell lines.
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The method adopted to map the DNA repair genes is based on making a hybrid between a microcell and an intact DNA repair deficient cell. In order to map the DNA repair genes, each normal human chromosome is transferred separately into the XP or CS DNA repair deficient cell lines. This is followed by the analysis of the transfer-clones for correction of the repair defect.
A panel of Mouse-Human monochromosomal hybrids is used as the source of normal human chromosomes. A typical panel should consist of 24 mouse cell lines each containing a single, marked human chromosome and represent all 22 autosomes and two human sex chromosomes.
The MMCT technique leads to the identification of the human chromosomes carrying the DNA repair genes. The identified chromosome is then fragmented for further mapping the gene location on the chromosome. Alternatively, commercially available panels of "Radiation Hybrids" can also be used for finer mapping.
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